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Run info:

Run Info: January - February, 2017

G1 Station:

E = 9.846 keV (1.259 Å)

beam diameter (250 µm x 250 µm)

Flux = 1.24 x 1012 photons/s (start of run)

Flux = 1.08 x 1012 photons/s (2/8)

Note: transient intensity drops by as much as 60% were observed roughly every 12 hr throughout this run. This problem was eventually located in the upstream beamline optics (a heat transfer issue) and resolved, but only at the end of the BioSAXS run. Since the change was very slow, it did n ot affect sample normalization and would be noticeable only as weaker-than-expected signals lasting few hours twice a day. You can check to see if you were affected by comparing the I1 counter values at various times located in the image header files. At start of run, I1 = 375000 counts/s.

Run Info: October - November, 2016

G1 Station:

E = 9.8528 keV (1.2584 Å)

beam diameter (250 µm x 250 µm)

November 11:

Flux = 8.4 x 1011 photons/s

November 4:

Flux = (am) 7.5 x 1011 photons/s
(re-optimized to 1.2 x 1012 photons/s based on beamstop reading)

October 29:

Flux = 1.1 x 1012 photons/s

October 26:

Flux = 6.5 x 1011 photons/s

 

Standards concentration

Lysozyme - 4.21 mg/ml ± 0.03 mg/ml

Glucose Isomerase - 0.33 mg/ml ± 0.004mg/ml

November 9:

Lysozyme - 4.00 mg/ml ± 0.01 mg/ml

 

Run Info: May - June, 2016

G1 Station:

Beam diameter 250 µm x 250 µm

May 21:

E = 9.781 keV (1.267 Å)

Flux = 7.76 x 1011 photons/s @ 124 ma

June 1:

E = 9.781 keV (1.267 Å)

Flux = 9.0 x 1011 photons/s @ 105 ma

 

Standards concentration

Lysozyme - 4.44 mg/ml ± 0.01 mg/ml

Glucose Isomerase - 0.44 mg/ml ± 0.004 mg/ml

 

Run Info: February - April, 2016

G1 Station:

Beam diameter 250 µm x 250 µm

Note: some SEC runs may have used 250 micron (V) by 500 micron (H) beam with approx 8 x 1011 photons/s flux)

March 16:

E = 9.822 keV (1.262 Å)

Flux = 4.35 x 1011 photons/s @ 100 ma

March 30:

E = 9.822 keV (1.262 Å)

Flux = 4.42 x 1011 photons/s @ 99 ma

April 1:

E = 9.822 keV (1.262 Å)

Flux = 3.26 x 1011 photons/s @ 99 ma

 

Standards concentration

Lysozyme - 4.12 mg/ml ± 0.03 mg/ml

Glucose Isomerase - 0.45 mg/ml ± 0.01mg/ml

 

Run Info: November - December, 2015

G1 Station:

E = 9.962 keV (1.245 Å)

Beam diameter 250 µm x 250 µm

Flux (photons/s) = 8.4 x 1011 @ 92.5 ma on 11/6

  • 7.3 x 1011 @ 92 ma 11/11
  • 3.6 x 1011 @ 92 ma 11/14
  • 5.9 x 1011 @ 92 ma on 11/18
  • 9.9 x 1011 @ 92 ma on 11/25 (500 µm x 250 µm)
  • 5.6 x 1011 @ 91.4 ma on 12/2

Note: we experienced flux variations from day to day during this run.

Beamstop counter readings (diode) in the image headers can be used to estimate actual flux. Conversion factor is 5.56 x 1011 photons/s per 222991 counts/s on beamstop diode.

Minimum sample volumes:
using the robot 25 L
manual loading 20 L
recommended working volume 30 L

Detector: dual Pilatus 100K-S SAXS/WAXS

q-space range:
qmin = 0.0075 - 0.28 -1
qmax = 0.22 - 0.8 -1

Recommended sample exposure: 10 frames @ 2 s each (20 s)

 

Standards concentration

Lysozyme - 4.27 mg/ml ± 0.01 mg/ml

Glucose Isomerase - 0.47 mg/ml ± 0.01mg/ml

 

Run Info: February 4 - 24, 2015

G1 Station:

E = 9.963 keV (1.244 Å)

Beam diameter 250 µm x 250 µm

Flux = 3.63 x 1011 photons/s @ 51 ma

Minimum sample volumes:
using the robot 25 L
manual loading 20 L
recommended working volume 30 L

Detector: dual Pilatus 100K-S SAXS/WAXS

q-space range:
qmin = 0.007 -1
qmax = 0.7 -1

Recommended sample exposure: 10 frames @ 2 s each (20 s)

 

Standards concentration

Lysozyme - 4.14 mg/ml ± 0.02 mg/ml

Glucose Isomerase - 0.577 mg/ml ± 0.01mg/ml

 

Run Info: October 8 - December 9, 2014

G1 Station: primary station for BioSAXS experiments

Note: this was a transitional period in the synchrotron upgrade plan that required running with a temporary 30 cm undulator at variable and lower than normal positron currents.

Energy = 11.75 keV (1.055 Å)

Beam diameter 250 µm x 500 µm

Detector: dual Pilatus 100K-S SAXS/WAXS

q-space range:
qmin = 0.007 -1
qmax = 0.7 -1

 

F1 Station: only used during BioSAXS workshops

Energy = 12.686 keV (0.97733 )

Detector = Dectris Pilatus 100k S  (SAXS only)

Sample-to-detector distance = 1140 mm

Beam diameter = 250 m x 250 m

Flux = 1.5 x 1011 photons/s

Minimum sample volumes:
using the robot 25 L
manual loading 20 L
recommended working volume 30 L

q_space range: 0.0164 to 0.45 -1
(using improved SAXS.cfg mask created after class end)

 

Standards concentration

Lysozyme - 4.33 mg/ml ± 0.02

Glucose Isomerase - 0.625 mg/ml ± 0.01

 

Past runs info

G1 Station:

E = 9.968 keV (1.257 ) (as of 10/18/2013)

Beam diameter 250 m x 250 m

Flux = 1.6 x 1011 photons/s (as of 10/18/2013)

Minimum sample volumes:
        using the robot 25 L
        manual loading  20 L
        recommended working volume 30 L

Detector: dual Pilatus 100K-S SAXS/WAXS

q-space range:
        qmin = 0.007 -1
        qmax = 0.7 -1

Recommended sample exposure: 10 frames @ 2 s each (20 s total).

 

F2 Station: E = 9.881 keV (decommissioned 6/13/2013)

Beam diameter: 250 m 250 m

Flux = 2 x 1010 photons/s

Minimum sample volumes:

using the robot: 15 L
manual loading: 5 L (practical limit)
recommended working volume: 30 L

Detector: dual Pilatus 100K-S SAXS/WAXS

q-space range:

qmin = 0.008 -1
qmax = 0.8 -1

Total exposure times: 300-600 s

 

Typical throughput: 80 sample plugs per 24 hours (about 20 protein samples including buffer, dilutions, and cleaning).