Oxford Cryosystems

home
 
   Getting Beam Time       chess : cuinfo : beam status : ithaca : weather  

 

 
About MacCHESS
MacCHESS People
Publications
Acknowledging MacCHESS
 
Beam lines
Beam Status
A1 beamline
F1 beamline
F2 beamline
 
Data collection
BioSAXS
Microcrystallography
High Pressure Cooling
MacCHESS automounter
Crystallography software
Data backup
How to
 
Technical information (primarily for staff)
Troubleshooting guide
BioSAXS Station Documentation
Crystal centering problems
Automounter problems
Improvements in adxv
Saving Images and Video
LED lighting
Small computers
 
 
 
  All three macromolecular stations have been equipped with new Cryostreams 700 series from Oxford Cryosystems.
 
  http://www.oxfordcryosystems.co.uk/cryo/cryostream/700series
 
  The temperature is usually set to 100 K, but can be varied from 80 K to 400 K. The systems can be programmed to cool down or warm up with a specific gradient, reach certain set points and hold these for a certain amount of time. 
 
  The cold gas flow is variable and can be set to either 5 or 10 L/min (turbo mode). For temperatures below 100 K the system automatically adjusts the flow to 10 L/min, for temperatures above 100 K a flow of 5L/min is sufficient to maintain the temperature within 0.1 K. The cold gas flow can be positioned in many different ways, from vertical to horizontal. 
 
  Accurate alignment is being done using the three positioners at the support stand with the aid of a nozzle alignment tool. This should be done with an empty pin or loop before mounting a crystal.
 
  Brief operation instructions are placed at the controller. They also contain some information about troubleshooting, but check first with the MacCHESS personnel on duty before attempting to fix a problem. 
 
 
Ulrich Englich
 
   

 

Last updated April, 2013
   
CHESS NIH NIGMS