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An experiment
is non-hazardous if: |
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the protein or other material
being studied is not toxic (or infectious) for humans;
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no heavy atom compounds are used
(intrinsic metals such as the Fe in hemoglobin are not considered as heavy
atoms, nor are metal foil reference samples);
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heavy atom compounds are present
only in pre-derivatized crystals.
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The crystals may be mounted in capillaries,
frozen, or contained in solutions free of the heavy atom compound.
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As long as you stay within this category,
CHESS does not need to be informed if you bring samples other than those
listed on the proposal. |
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An experiment
may be hazardous if: |
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Heavy atom compounds are present
outside of crystals.
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In this case, you
must supply CHESS with a Heavy Atom Compounds Declaration, listing the type
and amount of each compound. The Safety Committee will then classify the
experiment as hazardous or not, using the following guidelines
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For
solutions: |
The experiment is
considered non-hazardous if the total quantity of each heavy atom compound
is no more than 1/50 the LD-50 for a 50 kg. animal or 0.01 millimoles if the
LD-50 is not available. LD-50's, when known, are given on Material Safety
Data Sheets. If multiple values are given the most appropriate one (in terms
of method of administration and similarity of test animal to humans) is
used. If the total quantity is above these values but below 1/2 the LD-50
for a 50 kg. animal, or 0.25 mMoles if the LD-50 is not available, the
experiment is hazardous but may be carried out at CHESS. Quantities greater
than 1/2 the LD-50 or 0.25 mMoles may not be brought to CHESS.
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For dry
material: |
reference samples for MAD
experiments are non-hazardous if they are well-sealed, e.g. between two
layers of Kapton tape, before being brought to CHESS. For loose material,
the same limits on total quantity as for solutions are used.
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as hazardous, extra time will be
needed for the proposal to be processed. |
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An experiment
is hazardous if: |
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The protein or other material is
toxic or infectious to humans.
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In this case, you must supply
CHESS with a Hazardous Materials Declaration, and Viral Materials
Declaration, if appropriate. Extra time will be needed
to review this documentation. |
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