The Winter 2016 running period is upon us and we have lots of great new features and improvements! Flux at G1 reached 1.4 x 1012 photons/s at 125 ma in May 2016 and we anticipate further increases as CHESS-U shifts into high gear in the coming year. You can always find the latest beamline performance parameters on our website at http://www.macchess.cornell.edu/MacCHESS/beamline_character.html
BioSAXS setup at G1.
Manjie Huang has produced a nice document on optimizing your online size exclusion chromatography experiments at the beamline. We’ll be posting a link as soon as she’s finished testing the two new GE Superdex Increase columns that just arrived (5/150 and 10/300). We also have a new inline degasser for the SEC-SAXS system which should dramatically reduce any bubble formation! Check here for more details: www.macchess.cornell.edu/MacCHESS/inline_SEC.html
Jesse Hopkins has been very busy improving and expanding the RAW data reduction software. If you haven’t tried RAW, you’re in for a treat. RAW is a multi-platform, free, open source package that can run efficiently on a personal laptop. The software can handle some 27 different detector image formats (including PILATUS TIFF and CBF types), and has sophisticated masking and beamstop normalization capabilities. Beyond the requisite standard plots and Guinier analysis, RAW provides 4 different types of molecular weight estimates, including built-in absolute scale. Two different indirect Fourier transform methods are implemented (GNOM and BIFT), and the user interface is seamlessly integrated with the most widely used components of the popular ATSAS suite, including DAMMIF (shape reconstruction) and the new AMBIMETER program for determining shape ambiguity. RAW also supports the collection and processing of SEC-SAXS data. Look for more advanced processing features in this area soon! Full documentation, including a printable manual and YouTube videos can be found on SourceForge as well as on this website: sourceforge.net/projects/bioxtasraw.
Looking for new ways to solve aggregation problems? Try our new AirFuge. A number of users have successfully rescued aggregated samples by spinning aggressively at ultracentrifuge speeds for 1/2 hour.
TRAINING THIS YEAR: Don’t miss our upcoming BioSAXS Essentials course. We expect to offer this at the start of the June 2017 run, so stay tuned for an official announcement.
We just submitted our 5-year NIH renewal proposal. THANK YOU to all our wonderful users who have made this such a fantastic facility. You have been super productive in the past several years giving us a publication rate per beamtime day that is among the best in the nation. It has been a pleasure and an honor to serve you. In the new era of P30 funding we hope to continue our tradition of providing the best and most innovative BioSAXS for you.
-Richard Gillilan and Jesse Hopkins
How to apply for BioSAXS time
Users interested in trying BioSAXS may apply for time through the express-mode proposal mechanism. Visit: https://userdb.chess.cornell.edu/
BioSAXS Essentials 6 Workshop, May 2016,
YouTube, Web. 24 Aug. 2016.
- Acerbo, A. S., M. J. Cook and R. E. Gillilan (2015). "Upgrade of MacCHESS facility for X-ray scattering of biological macromolecules in solution." Journal of Synchrotron Radiation 22(1): 180-186
- Skou, S., R. E. Gillilan and N. Ando (2014). "Synchrotron-based small-angle X-ray scattering of proteins in solution." Nature Protocols 9(7): 1727-1739
- Gillilan R., C. M., Temnykh G., Møller M., Nielsen S. (2013). "CHIPS AND ROBOTS: SCREENING, MIXING, AND DIALYSIS ON BIOSAXS BEAMLINES." Transactions of the ACA 44: 40-50
- Nielsen, S. S., M. Moller and R. E. Gillilan (2012). "High-throughput biological small-angle X-ray scattering with a robotically loaded capillary cell." Journal of Applied Crystallography 45(2): 213-223
- Jacques, D. A. and J. Trewhella (2010). Small-angle scattering for structural biology — Expanding the frontier while avoiding the pitfalls. Protein Science 19(4): 642-657
- Putnam, C. D., M. Hammel, et al. (2007). X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution. Quarterly Reviews of Biophysics, 40(3): 191-285
- Mertens, H. D. T. and D. I. Svergun (2010). Structural characterization of proteins and complexes using small-angle X-ray solution scattering. Journal of Structural Biology, 172(1): 128-141
- Svergun, D. I. and M. H. J. Koch (2003). Small-angle scattering studies of biological macromolecules in solution. Reports on Progress in Physics, 66(10): 1735-1782